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Antony Joseph Buchala Former senior lecturer in
plant biochemistry & plant physiology Department of Biology
E-mail: antony.buchala@unifr.ch |
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Recent Publications
Complete list of publications
Picture gallery
Research Interests
Plant-pathogen interactions:
The plant cell wall:
External collaboration projects include:
Salicylic acid and oviposition (with P. Reymond, DMPB, University of Lausanne)
Salicylic acid, MAP kinase phosphatases and environmental stress signalling (R. Ulm and S. Bartels, Institute for Biology II, Albert-Ludwigs University, Freiburg)
Analysis of glucosinolates in the evolution of induced herbivore resistance in Sinapis arvensis (with T. Steinger, University of Fribourg)
Role of glucosinolates in defence against Spodoptera littoralis and microbial pathogens [with F. Mauch & K. Schlaeppi (Fribourg) and P. Reymond & N. Bodenhausen (Lausanne)]
Characterization of microbial communities in soil by fatty acid analysis (with M. Macel, University of Fribourg)
Adoposis and fatty acids in rat muscle tissue (with A. Dulloo, University of Fribourg)
Lecture courses
I taught the following lecture courses
1st year (Pharmacy): Plantes médicinales et vénéneuses: this course, designed for 1st year pharmacy students, was given in the summer semester comprises 1h lecture with 1h microscopy of plants of pharmaceutical interest. (I'm still on the job!).
2nd year (BSc): Biologie végétale I: this course, given in the winter semester was obligatory for 2nd-year students of biology and comprises 2h lectures and 2h work in the laboratory. The biochemical aspects of fundamental processes such as photosynthesis, anabolic and catabolic pathways of carbohydrate and lipid metabolism, and the assimilation of nitrogen are covered.
MSc: Metabolites important in health and survival: this course comprised 12h lectures in the winter semester and was obligatory for students studying plant biology. Although many of the molecules produced by plants have been attributed roles in planta, these molecules are also significant to our own life style. The biogenesis and role of these molecules in plants and the significance to man of some of the more important is discussed.
I still contrubute to the MSc plant metabolism lecture course at the University of Berne (BENEFRI).
University teaching project
A personal project
"Aider les étudiants à mieux comprendre" carried out as part of
the requirements for a university teaching course.
External activities
Editorial Board (until 2007), Journal of Experimental Botany
Expert
assessor for the Australian Research Council
Reviewer for the Biotechnology and Biological Science Research Council
(UK)
Reviewer for the Georgian National Science Foundation
University contact person for the Society for Experimental Biology (UK)
Webmaster for WINS
F. Bruessow, C. Gouhier-Darimont, A. Buchala, J.-P. Métraux and P. Reymond (2010). Insect eggs suppress plant defence against chewing herbivores . Plant Journal 62: 876-885.
K. Schläppi, E. Abou-Mansour, A. Buchala & F. Mauch (2010).
Disease resistance of Arabidopsis to Phytophthora brassicae is established by the sequential action of indole glucosinolates and camalexin. Plant Journal 62: 840-851.
K. Schlaeppi, E. Abou-Mansour, A. Buchala & F. Mauch (2010). Glucosinolates are an important first layer defence of Arabidopsis against Phytophthora brassicae. in "Biology of Molecular Plant-Microbe Interactions" Vol. 7 (CD-ROM), 2009 IS-MPMI Symposium Proceedings, eds. H. Antoun, L. Brisson, M. Trepanier, T. Avis & D. Prévost, APS Press.
S. Bartels, J.C. Anderson, M.A. Gonzalez Besteiro, A. Carreri, H. Hirt, A. Buchala, J.P. Métraux, S.C. peck & R.Ulm (2009). MAP KINASE PHOSPHATASE1 and PROTEIN TYROSINE PHOSPHATASE1 are repressors of salicylic acid and SNC1-mediated defence responses in Arabidopsis. Plant Cell 21: 2884-2897.
S. Summermatter, H. Marcelino, D. Arsenijevic, A. Buchala, O. Aprikian, F. Assimacopoulos-Jeannet, J. Seydoux, J.P. Montani, G. Solinas & A. Dulloo (2009). Adipose tissue plasticity during catch-up fat driven by thrifty metabolism: Relevance for muscle-adipose glucose redistribution during catch-up growth. Diabetes 58: 2228-2237.
F. Stefanato, E. Abou-Mansour, A. Buchala, M. Kretschmer, A. Mosbach, M. Hahn, C. Bochet, J.P. Metraux & H.J. Schoonbeek (2009). The ABC-transporter BcatrB from Botrytis cinerea exports camalexin and is a virulence factor on Arabidopsis thaliana. Plant Journal 58: 499-510.
A. Hukkanen, H. Kokko, A. Buchala, J. Häyrinen & S. Kärenlampi (2008). Benzothiadiazole affects the leaf proteome in arctic bramble (Rubus arcticus). Molecular Plant Pathology 9: 799-808.
C. Garcion, A. Lohmann, E. Lamodière, J. Catinot, A. Buchala, P. Doermann, & J.-P. Métraux (2008). Characterization and biological function of the ISOCHORISMATE SYNTHASE2 gene of Arabidopsis thaliana. Plant Physiology 147: 1279-1287.
K. Schlaeppi, N. Bodenhausen, A. Buchala, F. Mauch & P. Reymond (2008). The glutathione-deficient mutant pad2-1 accumulates lower amounts of glucosinolates and is more susceptible to the insect herbivore Spodoptera littoralis. Plant Journal 55: 774-786.
C. Chassot, A. Buchala, H.J. Schoonbeek, J.P. Métraux & O. Lamotte (2008): Wounding of Arabidopsis leaves causes a powerful but transient protection against Botrytis infection. Plant Journal 55: 555-567.
J. Catinot, A. Buchala, E. Abou-Mansour & J.P. Métraux (2008). Salicylic acid production in response to biotic and abiotic stress depends on isochorismate in Nicotiana benthamiana. FEBS Letters 582: 473-478.
S. Summermatter, D. Mainieri, A.P.
Russell, J. Seydoux, J.P. Montani, A. Buchala, G. Solinas & A.G.
Dulloo A.G (2008).
Thrifty metabolism that favours fat storage after caloric restriction:
a role for skeletal muscle phosphatidylinositol-3-kinase and
AMP-activated protein kinase. FASEB Journal 22:
774-785.
A. Schweighofer, V. Kazananviciute, E.Scheikl, M.Teige, R. Doczi, H. Hirt, M. Schwanninger, M. Kant, R. Schuurink, F. Mauch, A.J. Buchala, F. Cardinale & I. Meskiene (2007). PP2C Phosphatase AP2C1 controls MAPK, innate immunity and stress hormones in Arabidopsis. Plant Cell 19: 2213-2224.
A.T. Hukkanen, H.I. Kokko, A.J. Buchala, G.J. McDougal, D. Stewart, S.O. Kärenlampi & R.O. Karjalainen (2007). Benzthiadiazol induces the accumulation of phenolics and improves resistance to powdery mildew in strawberry. Journal of Agricultural and Food Chemistry 55: 1862-1870.
V. Parisy, B. Poinssot, L.
Owsianowski, A. Buchala, J. Glazebrook & F. Mauch (2007):
Identification of PAD2 as a gamma-glutamylcysteine synthetase
highlights the importance of glutathione in disease resistance of
Arabidopsis. Plant Journal 49:
159-172.
D. Mainieri , S. Summermatter, J.
Seydoux, S. Rusconi, A.P. Russell, J.P. Montani, O. Boss, A.J. Buchala
& A.G. Dulloo (2006): A role for skeletal muscle stearoyl-CoA
desaturase1 in the adipose-specific control of thermogenesis. FASEB
Journal 20: 1751-1753.
M. De Vos, V.R. Van Oosten, R.M.P. Van Poecke, J.A. Van Pelt, M.J. Pozo, M.J. Mueller, A.J. Buchala, J.-P. Métraux, L.C. Van Loon, M. Dicke & C.M.J. Pieterse (2005): Signal signature and transcriptome changes of Arabidopsis during pathogen and insect attack. Molecular Plant-Microbe Interactions 18: 923-937.
M. Cruz Castillo, C.
Martínez, A. Buchala, J.P. Métraux & J. León
(2004): Gene-specific involvement of beta-oxidation in wound-activated
responses in Arabidopsis thaliana. Plant Physiology 135:
85-94.
S. Heck, T. Grau, A.J. Buchala, J.P.Metraux & C. Nawrath (2003): Genetic evidence that expression of NahG modifies defence pathways independent of salicylic acid biosynthesis in the Arabidopsis - Pseudomonas syringae pv. tomato interaction. The Plant Journal 36: 342-352.
A. Iavicoli, E. Boutet, A. Buchala, Jean-Pierre Métraux (2003): Induced systemic resistance in Arabidopsis thaliana in response to root inoculation with Pseudomonas fluorescens CHA0. Molecular Plant-Microbe Interactions 16: 851-858.
S.H. Spoel, A. Koornneef, S.M.C. Claessens, J.P. Korzelius, J.A. Van Pelt, M.J. Mueller, A.J. Buchala, J.-P. Métraux, R. Brown, K. Kazan, L.C. Van Loon, X. Dong & C.M.J. Pieterse (2003): NPR1 modulates cross talk between salicylic acid- and jasmonate-dependent defense pathways through a novel function in the cytosol. Plant Cell 15: 760-770.
T. Genoud, A.J. Buchala, N.-H. Chua & J.P. Métraux (2002): Phytochrome signaling modulates the SA-perceptive pathway in Arabidopsis. The Plant Journal 31: 87-95.
J. Ton, M. De Vos, C. Robben, A.J. Buchala, J.P. Métraux, L.C. Van Loon & C.M.J. Pieterse (2002): Characterization of Arabidopsis enhanced disease susceptibility mutants that are affected in systemically induced resistance. The Plant Journal 26: 435-446.
S. Roulin, A.J. Buchala & G.B. Fincher (2002): Induction of (1,3/1,4)-beta-glucan hydrolases in dark-grown barley seedlings. Planta 215: 51-59.
J.-P. Métraux, A. Buchala & C. Nawrath (2001): Salicylic acid as a signal for systemic acquired resistance. In Polyphenols 98 (ed. A. Scalbert) INRA, pp xx-xx.
B. Hilpert, H. Bohlmann, R. op den Camp, D. Przybyla, O. Miersch, A. Buchala & K. Apel (2001): Isolation and characterization of signal transduction mutants of Arabidopsis thaliana that constituitively activate the octadecanoid pathway and form necrotic microlesions. The Plant Journal 26: 435-446.
C.M.J. Pieterse, J.A. van Pelt, J. Ton, S. Parchmann, M.J. Mueller, A.J. Buchala, J.-P. Métraux & L.C. van Loon (2000): Rhizobacteria-mediated induced systemic resistance (ISR) in Arabidopsis requires sensitivity to jasmonate and ethylene but is not accompanied by an increase in their production. Physiological and Molecular Plant Pathology 57: 123-134.
P. Sieber, M. Schorderet, U. Ryser, A. Buchala, P. Kolattukudy, J.P. Métraux & C. Nawrath (2000): Transgenic arabidopsis plants expressing a fungal cutinase show alterations in the structure and properties of the cuticle and postgenital organ fusions. Plant Cell 12: 721-737.
G. Kozlowski, A.J. Buchala & J.-P. Métraux (1999): Methyl jasmonate protects Norway spruce [Picea abies (L.) Karst] seedlings against Pythium ultimum Trow. Physiological and Molecular Plant Pathology 55: 53-58.
R. Schafleitner, A. Buchala & E. Wilhelm (1999): Class III chitinase expression and salicylic acid accumulation in chestnut after challenge with hypovirulent and virulent Cryphonectria parasitica murr.) barr. Phyton 39: 191-196.
G. DeMeyer, K. Capieau, K. Audenaert, A.J. Buchala, J.-P. Métraux & M. Höfte (1999): Nanogram amounts of salicylic acid produced by the rhizobacterium Pseudomonas aeruginosa 7NSK2 activate the systemic acquired resistance pathway in bean. Molecular Plant-Microbe Interactions 12: 450-458.
L. Moire, A. Schmutz, A. Buchala, B. Yan, R.E. Stark & U. Ryser (1999): Glycerol is a suberin monomer: New experimental evidence for an old hypothesis. Plant Physiology 119: 1137-1146.
A.J. Buchala (1999): Non-cellulosic carbohydrates in cotton fibers. Chap. 5 In "Cotton Fibres: Developmental Biology, Quality Improvement, and Textile Processing" (ed. A.S. Basra) HaworthPress, pp 113-136.
I.A.M.A. Penninckx, B.P.H.J. Thomma, A. Buchala, J.-P. Métraux & W.F. Broekaert (1998): Parallel but cooperative activation of jasmonate and ethylene response pathways is required for induction of a plant defensin gene in Arabidopsis. The Plant Cell 10: 2103-2114.
M. Fauth, P. Schweizer, A. Buchala, C. Marstädter, M. Riederer, T. Kato & H. Kauss (1998): Cutin monomers and surface wax constituents elicit H2O2 in conditioned cucumber hypocotyl segments and enhance the activity of other H2O2 elicitors. Plant Physiology 117: 1373-1380
J.L. Coquoz, A.J. Buchala & J.P. Métraux (1998):The biosynthesis of salicylic acid in potato. Plant Physiology 117: 1095-1101.
P. Schweizer, A. Buchala, R. Dudler & J.-P. Métraux (1998): Induced systemic resistance in wounded rice plants. The Plant Journal 14: 475-481.
P. Schweizer, A. Buchala & J.-P. Métraux (1997): Gene-expression patterns and levels of jasmonic acid in rice treated with the resistance inducer 2,6-dichloroisonicotinic acid. Plant Physiology 115: 61-70.
P. Schweizer, A. Buchala, P. Silverman, M. Seskar, I. Raskin & J.-P. Métraux (1997): Jasmonate- inducible genes are activated in rice by pathogen attack without a concomitant increase in endogenous jasmonate acid levels. Plant Physiology 114: 79-88.
I.A.M.A. Penninckx, K. Eggermont, F.R.G. Terras, B.P.H.J. Thomma, G.W. De Samblanx, J.M. Manners, A. Buchala, J.-P. Métraux & W.F. Broekaert (1996): Pathogen-induced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic acid-independent pathway. The Plant Cell 8: 2309-2323.
W. Mölders, Buchala, A.J. & Métraux, J.P. (1996): Transport of salicylic acid in tobacco necrosis virus infected cucumber plants. Plant Physiology 112: 787-792.
Schmutz, A.J. Buchala & U. Ryser (1996): Changing the dimensions of suberin lamellae of green cotton fibers with a specific inhibitor of endoplasmic reticulum-associated fatty acid elongases. Plant Physiology 110: 403-411.
P. Schweizer, G. Felix, A.J. Buchala, C. Müller & J.-P. Métraux (1996): Perception of free cutin monomers by plant cells. The Plant Journal 10: 331-341.
Plantes médicinales
et vénéneuses
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This course is designed to give pharmacy students their first taste of pharmacy. A simple phytochemical-based approach to pharmacognosy is used to introduce the different types of plant natural products of medicinal importance and their use or abuse is explained. The different chapters cover alcaloids, anthrone glycosides, cardioactive glycosides, coumarins, cyanogenic glycosides, essential oils, flavonoid glycosides, glucosinolates, steroidal saponins, and tanins. Wild plants typical of Switzerland that contain such natural products are examined and their therapeutic value or eventual toxicity are discussed. The course is accompanied by practical work where basic features of the plants are examined under the microscope. In the same semester the students attend lectures on plant systematics and a practical course on the identification of wild plants found in Switzerland. The plant shown is Gentiana lutea (gentian) an essential ingredient of traditional bitter beverages. The bitter constituents (iridoids, e.g. amarogentin) stimulate gastric secretion helping digestion. Medicinally, gentian strengthens a weak or underactive digestive system |
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Biologie
végétale I
The biochemical aspects of the fundamental processes involved in plant
primary metabolism are discussed. Emphasis is placed on
The lecture course is accompanied by a weekly afternoon in the laboratory where the students familiarize themselves with standard laboratory equipment and apparatus in carrying out practical exercises that relate to the week's lecture.
Metabolites
important in health and survival
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Life could be really difficult for this plant! It has to start and continue to grow, be attractive to insects or birds for pollination and seed dispersal and survive for the next season. These requirements are often contradictory, e.g. attracting insects leads to the laying of eggs that produce larvae that consume the plant. Plants have to develop both general and specific solutions for potentially advantageous or disadvantageous interactions with their environment in order to survive. Secondary metabolites (natural products) can play a significant role in such interactions. Brightly coloured colours and particular odours can attract insects while the leaves can contain toxic or deterrent substances. Certain butterflies profit from the fact that such substances, although not toxic to the larvae, are toxic to predators of the larvae. Many of these substances are also important to our own health and life style. The lecture course focusses on the biochemical pathways leading to the synthesis of natural products and the recognition of any such pathway upon studying the chemical structure of a natural product. The different types of pigments (flavonoids, xanthones, quinones, carotenoids), odours (monoterpenes, sesquiterpenes), bitter compounds (tanins, alcaloids, terpenes), plant defence compounds (simple phenols, lignin, tanins, terpenes), plant growth substances (auxins, gibberellins, abscisic acid) and plant signalling molecules (salicylic acid, jasmonic acid) are discussed. |
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 Summertime when the cotton was high |
 The leaf on
the right was from a potato plant treated with |
more images can be
found at the links below
The Department past & present
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21st January 2012
